Microbial diversity and stability within Gut³Gel

Key Takeaways

The Shannon index values in Gut³Gel remained relatively stable across donor samples, despite notable reductions in diversity in some cases, indicating a shift in the microbial community between the initial timepoint (0 h) and later timepoints (24, 48, and 72 h).

The decrease in diversity indicates that culturing in Gut³Gel may be leading to significant alterations in the abundance of specific taxa when compared to the initial inoculum.

Beta diversity analysis shows a significant departure of Gut³Gel from the planktonic condition (BHI medium) in terms of dissimilarity both based on abundance and phylogeny (weighted UniFrac) across all donors (PERMANOVA, p < 0.05).

Note

Representative results of beta diversity are displayed. If you are interested in the full data please refer to our preprint https://www.biorxiv.org/content/10.1101/2025.02.21.639490v1