Taxonomic composition of intestinal microbiota cultured in Gut³Gel

Key Takeways:

Firmicutes is the most abundant taxa in both Gut³Gel and BHI

Actinobacteriota is substantially higher in Gut³Gel (up to 2.5-fold), correponding mainly to Bifidobacterium and Collinsella

Proteobacteria (mainly facultative anaerobes like Escherichia and Pseudomonas) is 3- to 9.5-fold lower in Gut³Gel, demonstrating that Gut³Gel does not require anaerobic conditions

Note

The rose charts display representative results for one Donor sample. If you are interested in the full data please refer to our preprint https://www.biorxiv.org/content/10.1101/2025.02.21.639490v1

Key Takeaways

Species belonging to the Families Bacteroidaceae, Coriobacteriaceae, Prevotellaceae, Lachnospiraceae, Oscillospiraceae, and Ruminococcaceae (the latter 3 being mucosal-associated) consistently exhibited a substantially high abundance across all donors in Gut³Gel, with a centered log-ratio (CLR) value above 2.

The high variability in CLR abundance of species from several Families, as evidenced by the distinct microbial abundance profiles across donors, underscores the ability of Gut³Gel to preserve interindividual differences among donors.